Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SUPT20HL2 / FAM48B2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G125706-100
Catalog Number: LS-G125706
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SUPT20HL2 (NM_001136233), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Origin Species
- Human
- NCBI GenBank
- NM_001136233
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 87.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDRDLEQALD RTENITEIAQ QRRPRRRYSP RAGKTLQEKL YDIYVEECGK EPEDPQELRS61NVNLLEKLVR RESLPCLLVN LYPGNQGYSV MLQREDGSFA ETIRLPYEER ALLDYLDAEE121LPPALGDVLD KASVNIFHSG CVIVEVRDYR QSSNMQPPGY QSRHILLRPT MQTLAPEVKT181MTRDGEKWSQ EDKFPLESQL ILATAEPLCL DPSVAVACTA NRLLYNKQKM NTDPMEQCLQ241RYSWPSVKPQ QEQSDCPPPP ELRVSTSGQK EERKVGQPCE LNITKAGSCV DTWKGRPCDL301AVPSEVDVEK LAKGYQSVTA ADPQLPVWPA QEVEDPFRHA WEAGCQAWDT KPNIMQSFND361PLLCGKIRPR KKARQKSQKS PWQPFPDDHS ACLRPGSETD AGRAVSQAQE SVQSKVKGPG421KMSHSSSGPA SVSQLSSWKT PEQPDPVWVQ SSVSGKGEKH PPPRTQLPSS SGKISSGNSF481PPQQAGSPLK RPFPAAAPAV AAAAPAPAPA PAAAPALAAA AVAAAAGGAA PSHSQKPSVP541LIKASRRRPA AGRPTRFVKI APAIQVRTGS TGLKATNVEG PVRGAQVLGC SFKPVQAPGS601GAPAPAGISG SGLQSSGGPL PDARPGAVQA SSPAPLQFFL NTPEGLRPLT LQVPQGWAVL661TGPQQQSHQL VSLQQLQQPT AAHPPQPGPQ GSTLGLSTQG QAFPAQQLLN VNLTGAGSGL721QPQPQAAVLS LLGSAQVPQQ GVQLPFVLGQ QPQPLLLLQP QPQPQQIQLQ TQPLRVLQQP781VFLATGAVQI VQPHPGVQAG SQLVGQRKGG KPTPPAPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
817 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.