Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SUNC1 / SUN3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G120787-100
Catalog Number: LS-G120787
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SUNC1(SUN3) (NM_001030019), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Hypothetical protein MGC33329 | SUN3 | SUNC1
- Origin Species
- Human
- NCBI GenBank
- AA973064
- SwissProt
- Q8TAQ9
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 40.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSGKTKARRA AMFFRRCSED ASGSASGNAL LSEDENPDAN GVTRSWKIIL STMLTLTFLL61VGLLNHQWLK ETDVPQKSRQ LYAIIAEYGS RLYKYQARLR MPKEQLELLK KESQNLENNF121RQILFLIEQI DVLKALLRDM KDGMDNNHNW NTHGDPVEDP DHTEEVSNLV NYVLKKLRED181QVEMADYALK SAGASIIEAG TSESYKNNKA KLYWHGIGFL NHEMPPDIIL QPDVYPGKCW241AFPGSQGHTL IKLATKIIPT AVTMEHISEK VSPSGNISSA PKEFSVYGIT KKCEGEEIFL301GQFIYNKTGT TVQTFELQHA VSEYLLCVKL NIFSNWGHPK YTCLYRFRVH GTPGKHITRTRPLEQKLISEEDLAANDILDYKDDDDKV
357 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.