Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SUGP2 / SFRS14 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G121308-100
Catalog Number: LS-G121308
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SFRS14(SUGP2) (NM_001017392), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- SFRS14 | SRFS14 | SUGP2 | KIAA0365
- Origin Species
- Human
- NCBI GenBank
- NM_001017392
- SwissProt
- Q8IX01
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 120 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAARRITQET FDAVLQEKAK RYHMDASGEA VSETLQFKAQ DLLRAVPRSR AEMYDDVHSD61GRYSLSGSVA HSRDAGREGL RSDVFPGPSF RSSNPSISDD SYFRKECGRD LEFSHSDSRD121QVIGHRKLGH FRSQDWKFAL RGSWEQDFGH PVSQESSWSQ EYSFGPSAVL GDFGSSRLIE181KECLEKESRD YDVDHPGEAD SVLRGGSQVQ ARGRALNIVD QEGSLLGKGE TQGLLTAKGG241VGKLVTLRNV STKKIPTVNR ITPKTQGTNQ IQKNTPSPDV TLGTNPGTED IQFPIQKIPL301GLDLKNLRLP RRKMSFDIID KSDVFSRFGI EIIKWAGFHT IKDDIKFSQL FQTLFELETE361TCAKMLASFK CSLKPEHRDF CFFTIKFLKH SALKTPRVDN EFLNMLLDKG AVKTKNCFFE421IIKPFDKYIM RLQDRLLKSV TPLLMACNAY ELSVKMKTLS NPLDLALALE TTNSLCRKSL481ALLGQTFSLA SSFRQEKILE AVGLQDIAPS PAAFPNFEDS TLFGREYIDH LKAWLVSSGC541PLQVKKAEPE PMREEEKMIP PTKPEIQAKA PSSLSDAVPQ RADHRVVGTI DQLVKRVIEG601SLSPKERTLL KEDPAYWFLS DENSLEYKYY KLKLAEMQRM SENLRGADQK PTSADCAVRA661MLYSRAVRNL KKKLLPWQRR GLLRAQGLRG WKARRATTGT QTLLSSGTRL KHHGRQAPGL721SQAKPSLPDR NDAAKDCPPD PVGPSPQDPS LEASGPSPKP AGVDISEAPQ TSSPCPSADI781DMKTMETAEK LARFVAQVGP EIEQFSIENS TDNPDLWFLH DQNSSAFKFY RKKVFELCPS841ICFTSSPHNL HTGGGDTTGS QESPVDLMEG EAEFEDEPPP REAELESPEV MPEEEDEDDE901DGGEEAPAPG GAGKSEGSTP ADGLPGEAAE DDLAGAPALS QASSGTCFPR KRISSKSLKV961GMIPAPKRVC LIQEPKVHEP VRIAYDRPRG RPMSKKKKPK DLDFAQQKLT DKNLGFQMLQ1021KMGWKEGHGL GSLGKGIREP VSVGTPSEGE GLGADGQEHK EDTFDVFRQR MMQMYRHKRA1081NKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1082 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.