LSBio
SUGCT / C7orf10 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SUGCT / C7orf10 Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all SUGCT antibodies →
$725.00each
Catalog Number: LS-G109815-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C7orf10(SUGCT) (NM_024728), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C7orf10 | DERP13 | ORF19
Origin Species
Human
NCBI GenBank
NM_024728
SwissProt
Q9HAC7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
47 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPSETHAMLA TLARVAALRR TCLFSGRGGG RGLWTGRPQS DMNNIKPLEG VKILDLTRVL
61AGPFATMNLG DLGAEVIKVE RPGAGDDTRT WGPPFVGTES TYYLSVNRNK KSIAVNIKDP
121KGVKIIYCSI TGYGQTGPIS QRAGYDAVAS AVSGLMHITG PENGDPVRPG VAMTDLATGL
181YAYGAIMAGL IQKYKTGKGL FIDCNLLSSQ VACLSHIAAN YLIGQKEAKR WGTAHGSIVP
241YQAFKTKDGY IVVGAGNNQQ FATVCKILDL PELIDNSKYK TNHLRVHNRK ELIKILSERF
301EEELTSKWLY LFEGSGVPYG PINNMKNVFA EPQNAVSGFQ SLLHSLAHGP FLHLQGSARV
361LHNGLVMEME HPTVGKISVP GPAVRYSKFK MSEARPPPLL GQHTTHILKE VLRYDDRAIG
421ELLSAGVVDQ HETH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

434 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.