Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SUCNR1 / GPR91 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G102101-100
Catalog Number: LS-G102101
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GPR91(SUCNR1) (NM_033050), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- G protein-coupled receptor 91 | GPR91 | p2Y purinoceptor 1-like | p2Y purinoceptor 1 | Succinate receptor 1 | G-protein coupled receptor 91 | p2u2 | SUCNR1
- Origin Species
- Human
- NCBI GenBank
- NM_033050
- SwissProt
- Q9BXA5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 38.5 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MLGIMAWNAT CKNWLAAEAA LEKYYLSIFY GIEFVVGVLG NTIVVYGYIF SLKNWNSSNI61YLFNLSVSDL AFLCTLPMLI RSYANGNWIY GDVLCISNRY VLHANLYTSI LFLTFISIDR121YLIIKYPFRE HLLQKKEFAI LISLAIWVLV TLELLPILPL INPVITDNGT TCNDFASSGD181PNYNLIYSMC LTLLGFLIPL FVMCFFYYKI ALFLKQRNRQ VATALPLEKP LNLVIMAVVI241FSVLFTPYHV MRNVRIASRL GSWKQYQCTQ VVINSFYIVT RPLAFLNSVI NPVFYFLLGD301HFRDMLMNQL RHNFKSLTSF SRWAHELLLS FREKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
334 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.