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STYK1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

STYK1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G101568-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human STYK1 (NM_018423), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
NOK | Protein PK-unique | SuRTK106 | STYK1 | Tyrosine-protein kinase STYK1
Origin Species
Human
NCBI GenBank
NM_018423
SwissProt
Q6J9G0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
47.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGMTRMLLEC SLSDKLCVIQ EKQYEVIIVP TLLVTIFLIL LGVILWLFIR EQRTQQQRSG
61PQGIAPVPPP RDLSWEAGHG GNVALPLKET SVENFLGATT PALAKLQVPR EQLSEVLEQI
121CSGSCGPIFR ANMNTGDPSK PKSVILKALK EPAGLHEVQD FLGRIQFHQY LGKHKNLVQL
181EGCCTEKLPL YMVLEDVAQG DLLGFLWTCR RDVMTMDGLL YDLTEKQVYH IGKQVLLALE
241FLQEKHLFHG DVAARNILMQ SDLTAKLCGL GLAYEVYTRG AISSTQTIPL KWLAPERLLL
301RPASIRADVW SFGILLYEMV TLGAPPYPEV PPTSILEHLQ RRKIMKRPSS CTHTMYSIMK
361SCWRWREADR PSPRELRLRL EAAIKTADDE AVLQVPELVV PELYAAVAGI RVESLFYNYS
421ML
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

422 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.