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STXBP4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

STXBP4 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G104645-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Syntaxin binding protein 4(STXBP4) (NM_178509), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
STXBP4 | Synip | Syntaxin 4 interacting protein | Syntaxin 4-interacting protein | STX4-interacting protein | Syntaxin-binding protein 4 | Syntaxin binding protein 4
Origin Species
Human
SwissProt
Q6ZWJ1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
61.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNKNTSTVVS PSLLEKDPAF QMITIAKETG LGLKVLGGIN RNEGPLVYIQ EIIPGGDCYK
61DGRLKPGDQL VSVNKESMIG VSFEEAKSII TGAKLRLESA WEIAFIRQKS DNIQPENLSC
121TSLIEASGEY GPQASTLSLF SSPPEILIPK TSSTPKTNND ILSSCEIKTG YNKTVQIPIT
181SENSTVGLSN TDVASAWTEN YGLQEKISLN PSVRFKAEKL EMALNYLGIQ PTKEQHQALR
241QQVQADSKGT VSFGDFVQVA RNLFCLQLDE VNVGAHEISN ILDSQLLPCD SSEADEMERL
301KCERDDALKE VNTLKEKLLE SDKQRKQLTE ELQNVKQEAK AVVEETRALR SRIHLAEAAQ
361RQAHGMEMDY EEVIRLLEAK ITELKAQLAD YSDQNKESVQ DLKKRIMVLD CQLRKSEMAR
421KTFEASTEKL LHFVEAIQEV FSDNSTPLSN LSERRAVLAS QTSLTPLGRN GRSIPATLAL
481ESKELVKSVR ALLDMDCLPY GWEEAYTADG IKYFINHVTQ TTSWIHPVMS VLNLSRSEEN
541EEDCSRELPN QKS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

553 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.