Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
STX18 / Syntaxin 18 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G112423-100
Catalog Number: LS-G112423
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Syntaxin 18(STX18) (NM_016930), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Syntaxin 18 | Syntaxin-18 | STX18 | Ufe1
- Origin Species
- Human
- NCBI GenBank
- NM_016930
- SwissProt
- Q9P2W9
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 38.5 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MAVDITLLFR ASVKTVKTRN KALGVAVGGG VDGSRDELFR RSPRPKGDFS SRAREVISHI61GKLRDFLLEH RKDYINAYSH TMSEYGRMTD TERDQIDQDA QIFMRTCSEA IQQLRTEAHK121EIHSQQVKEH RTAVLDFIED YLKRVCKLYS EQRAIRVKRV VDKKRLSKLE PEPNTKTRES181TSSEKVSQSP SKDSEENPAT EERPEKILAE TQPELGTWGD GKGEDELSPE EIQMFEQENQ241RLIGEMNSLF DEVRQIEGRV VEISRLQEIF TEKVLQQEAE IDSIHQLVVG ATENIKEGNE301DIREAIKNNA GFRVWILFFL VMCSFSLLFL DWYDSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
335 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.