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STRN3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

STRN3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all STRN3 antibodies →
$725.00each
Catalog Number: LS-G113859-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Striatin3(STRN3) (NM_014574), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
GS2NA | Nuclear autoantigen | S/G2 antigen | Striatin-3 | SG2NA | STRN3 | Cell cycle autoantigen SG2NA
Origin Species
Human
NCBI GenBank
NM_014574
SwissProt
Q13033

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
77.6 kda
Protein Type
Lysate
Amino Acid Sequence
1MDELAGGGGG GPGMAAPPRQ QQGPGGNLGL SPGGNGAAGG GGPPASEGAG PAAGPELSRP
61QQYTIPGILH YIQHEWARFE MERAHWEGER AELQARIAFL QGERKGQENL KKDLVRRIKM
121LEYALKQERA KYHKLKYGTE LNQGDLKMPT FESEETKDTE APTAPQNSQL TWKQGRQLLR
181QYLQEVGYTD TILDVRSQRV RSLLGLSNSE PNGSVETKNL EQILNGGESP KQKGQEIKRS
241SGDVLETFNF LENADDSDED EENDMIEGIP EGKDKHRMNK HKIGNEGLAA DLTDDPDTEE
301ALKEFDFLVT AEDGEGAGEA RSSGDGTEWA EPITFPSGGG KSFIMGSDDV LLSVLGLGDL
361ADLTVTNDAD YSYDLPANKD AFRKTWSPKY TLRSHFDGVR ALAFHPVEPV LVTASEDHTL
421KLWNLQKTVP AKKSASLDVE PIYTFRAHIG PVLSLAISSN GEQCFSGGID ATIQWWNMPS
481PSVDPYDTYE PNVLAGTLVG HTDAVWGLAY SGIKNQLLSC SADGTVRLWN PQEKLPCICT
541YNGDKKHGIP TSVDFIGCDP AHMVTSFNTG SAVIYDLETS QSLVILSSQV DSGLQSNNHI
601NRVVSHPTLP VTITAHEDRH IKFFDNKTGK MIHSMVAHLD AVTSLAVDPN GIYLMSGSHD
661CSIRLWNLDS KTCVQEITAH RKKLDESIYD VAFHSSKAYI ASAGADALAK VFV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

713 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.