Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
STRADA / LYK5 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G122488-100
Catalog Number: LS-G122488
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LYK5(STRADA) (NM_001003788), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Protein kinase LYK5 | LYK5 | Stlk | STRADA | NY-BR-96 | PMSE | STE20-like pseudokinase | STE20-related adapter protein | STRAD | STRAD alpha
- Origin Species
- Human
- NCBI GenBank
- NM_001003787
- SwissProt
- Q7RTN6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 41.5 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MSSFLPEGGC YELLTVIGKG FEDLMTVNLA RYKPTGEYVT VRRINLEACS NEMVTFLQGE61LHVSKLFNHP NIVPYRATFI ADNELWVVTS FMAYGSAKDL ICTHFMDGMN ELAIAYILQG121VLKALDYIHH MGYVHRSVKA SHILISVDGK VYLSGLRSNL SMISHGQRQR VVHDFPKYSV181KVLPWLSPEV LQQNLQGYDA KSDIYSVGIT ACELANGHVP FKDMPATQML LEKLNGTVPC241LLDTSTIPAE ELTMSPSRSV ANSGLSDSLT TSTPRPSNGD SPSHPYHRTF SPHFHHFVEQ301CLQRNPDARP SASTLLNHSF FKQIKRRASE ALPELLRPVT PITNFEGSQS QDHSGIFGLV361TNLEELEVDD WEFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
373 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.