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STON1-GTF2A1L Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

STON1-GTF2A1L Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G105436-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SALF(STON1-GTF2A1L) (NM_172311), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_172311

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
131.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MCSTNPGKWV TFDDDPAVQS SQKSKNFPLE NQGVCRPNGL KLNLPGLREF PSGSSSTSST
61PLSSPIVDFY FSPGPPSNSP LSTPTKDFPG FPGIPKAGTH VLYPIPESSS DSPLAISGGE
121SSLLPTRPTC LSHALLPSDH SCTHPTPKVG LPDEVNPQQA ESLGFQSDDL PQFQYFREDC
181AFSSPFWKDE GSDSHFTLDP PGSKKMFSSR NKEMPIDQKS LNKCSLNYIC EKLEHLQSAE
241NQDSLRSLSM HCLCAEENAS SFVPHTLFRS QPKSGWSFML RIPEKKNMMS SRQWGPIFLK
301VLPGGILQMY YEQGLEKPFK EIQLDPYCRL SEPKVENFSV AGKIHTVKIE HVSYTEKRKY
361HSKTEVVHEP DIEQMLKLGS TSYHDFLDFL TTVEEELMKL PAVSKPKKNY EEQEISLEIV
421DNFWGKVTKE GKFVESAVIT QIYCLCFVNG NLECFLTLND LELPKRDESY YEKDSEKKGI
481DILDYHFHKC VNVQEFEQSR IIKFVPLDAC RFELMRFKTL YNGDNLPFSL KSVVVVQGAY
541VELQAFVNMA SLAQRSSYAG SLRSCDNIRI HFPVPSQWIK ALWTMNLQRQ KSLKAKMNRR
601ACLGSLHELE SEPVIQVTVG SAKYESAYQA VVWKIDRLPD KNSSLDHPHC LSYKLELGSD
661QEIPSDWYPF ATVQFSVPDT CASRTEVRSL GVESDVQPQK HVQQRACYNI QPKLYRSVIE
721DVIEGVRNLF AEEGIEEQVL KDLKQLWETK VLQSKATEDF FRNSIQSPLF TLQLPHSLHQ
781TLQSSTASLV IPAGRTLPSF TTAELGTSNS SANFTFPGYP IHVPAGVTLQ TVSGHLYKVN
841VPIMVTETSG RAGILQHPIQ QVFQQLGQPS VIQTSVPQLN PWSLQATTEK SQRIETVLQQ
901PAILPSGPVD RKHLENATSD ILVSPGNEHK IVPEALLCHQ ESSHYISLPG VVFSPQVSQT
961NSNVESVLSG SASMAQNLHD ESLSTSPHGA LHQHVTDIQL HILKNRMYGC DSVKQPRNIE
1021EPSNIPVSEK DSNSQVDLSI RVTDDDIGEI IQVDGSGDTS SNEEIGSTRD ADENEFLGNI
1081DGGDLKVPEE EADSISNEDS ATNSSDNEDP QVNIVEEDPL NSGDDVSEQD VPDLFDTDNV
1141IVCQYDKIHR SKNKWKFYLK DGVMCFGGRD YVFAKAIGDA EW
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1182 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.