Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
STK39 / SPAK Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G101111-100
Catalog Number: LS-G101111
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human STK39 (NM_013233), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Serine threonine kinase 39 | STE20/SPS1 homolog | STK39 | Ste20-like protein kinase | DCHT | SPAK | Ste-20-related kinase
- Origin Species
- Human
- NCBI GenBank
- NM_013233
- SwissProt
- Q9UEW8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 59.3 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MAEPSGSPVH VQLPQQAAPV TAAAAAAPAA ATAAPAPAAP AAPAPAPAPA PAAQAVGWPI61CRDAYELQEV IGSGATAVVQ AALCKPRQER VAIKRINLEK CQTSMDELLK EIQAMSQCSH121PNVVTYYTSF VVKDELWLVM KLLSGGSMLD IIKYIVNRGE HKNGVLEEAI IATILKEVLE181GLDYLHRNGQ IHRDLKAGNI LLGEDGSVQI ADFGVSAFLA TGGDVTRNKV RKTFVGTPCW241MAPEVMEQVR GYDFKADMWS FGITAIELAT GAAPYHKYPP MKVLMLTLQN DPPTLETGVE301DKEMMKKYGK SFRKLLSLCL QKDPSKRPTA AELLKCKFFQ KAKNREYLIE KLLTRTPDIA361QRAKKVRRVP GSSGHLHKTE DGDWEWSDDE MDEKSEEGKA AFSQEKSRRV KEENPEIAVS421ASTIPEQIQS LSVHDSQGPP NANEDYREAS SCAVNLVLRL RNSRKELNDI RFEFTPGRDT481ADGVSQELFS AGLVDGHDVV IVAANLQKIV DDPKALKTLT FKLASGCDGS EIPDEVKLIG541FAQLSVSSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
547 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.