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STK35 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

STK35 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all STK35 antibodies →
$504.00each
Catalog Number: LS-G129028-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human STK35 (NM_080836), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BA550O8.2 | CLIK-1 | CLP-36-interacting kinase 1 | CLP-36 interacting kinase | Serine/threonine kinase 35 | STK35L1 | PDIK1 | PDLIM1-interacting kinase 1 | CLIK1 | STK35
Origin Species
Human
NCBI GenBank
NM_080836
SwissProt
Q8TDR2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
57.9 kda
Protein Type
Lysate
Amino Acid Sequence
1MGHQESPLAR APAGGAAYVK RLCKGLSWRE HVESHGSLGA QASPASAAAA EGSATRRARA
61ATSRAARSRR QPGPGADHPQ AGAPGGKRAA RKWRCAGQVT IQGPAPPRPR AGRRDEAGGA
121RAAPLLLPPP PAAMETGKDG ARRGTQSPER KRRSPVPRAP STKLRPAAAA RAMDPVAAEA
181PGEAFLARRR PEGGGGSARP RYSLLAEIGR GSYGVVYEAV AGRSGARVAV KKIRCDAPEN
241VELALAEFWA LTSLKRRHQN VVQFEECVLQ RNGLAQRMSH GNKSSQLYLR LVETSLKGER
301ILGYAEEPCY LWFVMEFCEG GDLNQYVLSR RPDPATNKSF MLQLTSAIAF LHKNHIVHRD
361LKPDNILITE RSGTPILKVA DFGLSKVCAG LAPRGKEGNQ DNKNVNVNKY WLSSACGSDF
421YMAPEVWEGH YTAKADIFAL GIIIWAMIER ITFIDSETKK ELLGTYIKQG TEIVPVGEAL
481LENPKMELHI PQKRRTSMSE GIKQLLKDML AANPQDRPDA FELETRMDQV TCAA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

534 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.