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STK31 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

STK31 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all STK31 antibodies →
$504.00each
Catalog Number: LS-G109232-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human STK31 (NM_031414), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
SGK396 | Serine/threonine kinase 31 | Sugen kinase 396 | STK31 | TDRD8 | Tudor domain containing 8
Origin Species
Human
NCBI GenBank
NM_031414
SwissProt
Q9BXU1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
115.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MWVQGHSSRA SATESVSFSG IVQMDEDTHY DKVEDVVGSH IEDAVTFWAQ SINRNKDIMK
61IGCSLSEVCP HASSVLGNLD PNKIYGGLFS EDQCWYRCKV LKIISVEKCL VRYIDYGNTE
121ILNRSDIVEI PLELQFSSVA KKYKLWGLHI PSDQEVTQFD QGTTFLGSLI FEKEIKMRIK
181ATSEDGTVIA QAEYGSVDIG EEVLKKGFAE KCRLASRTDI CEEKKLDPGQ LVLRNLKSPI
241PLWGHRSNQS TFSRPKGHLS EKMTLDLKDE NDAGNLITFP KESLAVGDFN LGSNVSLEKI
301KQDQKLIEEN EKLKTEKDAL LESYKALELK VEQIAQELQQ EKAAAVDLTN HLEYTLKTYI
361DTRMKNLAAK MEILKEMRHV DISVRFGKDL SDAIQVLDEG CFTTPASLNG LEIIWAEYSL
421AQENIKTCEY VSEGNILIAQ RNEMQQKLYM SVEDFILEVD ESSLNKRLKT LQDLSVSLEA
481VYGQAKEGAN SDEILKKFYD WKCDKREEFT SVRSETDASL HRLVAWFQRT LKVFDLSVEG
541SLISEDAMDN IDEILEKTES SVCKELEIAL VDQGDADKEI ISNTYSQVLQ KIHSEERLIA
601TVQAKYKDSI EFKKQLIEYL NKSPSVDHLL SIKKTLKSLK ALLRWKLVEK SNLEESDDPD
661GSQIEKIKEE ITQLRNNVFQ EIYHEREEYE MLTSLAQKWF PELPLLHPEI GLLKYMNSGG
721LLTMSLERDL LDAEPMKELS SKRPLVRSEV NGQIILLKGY SVDVDTEAKV IERAATYHRA
781WREAEGDSGL LPLIFLFLCK SDPMAYLMVP YYPRANLNAV QANMPLNSEE TLKVMKGVAQ
841GLHTLHKADI IHGSLHQNNV FALNREQGIV GDFDFTKSVS QRASVNMMVG DLSLMSPELK
901MGKPASPGSD LYAYGCLLLW LSVQNQEFEI NKDGIPKVDQ FHLDDKVKSL LCSLICYRSS
961MTAEQVLNAE CFLMPKEQSV PNPEKDTEYT LYKKEEEIKT ENLDKCMEKT RNGEANFDC
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1019 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.