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STIM2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

STIM2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all STIM2 antibodies →
$725.00each
Catalog Number: LS-G101692-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human STIM2 (NM_020860), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1482 | Stromal interaction molecule 2 | STIM2
Origin Species
Human
NCBI GenBank
NM_020860

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
82.6 kda
Protein Type
Lysate
Amino Acid Sequence
1MNAAGIRAPE AAGADGTRLA PGGSPCLRRR GRPEESPAAV VAPRGAGELQ AAGAPLRFHP
61ASPRRLHPAS TPGPAWGWLL RRRRWAALLV LGLLVAGAAD GCELVPRHLR GRRATGSAAT
121AASSPAAAAG DSPALMTDPC MSLSPPCFTE EDRFSLEALQ TIHKQMDDDK DGGIEVEESD
181EFIREDMKYK DATNKHSHLH REDKHITIED LWKRWKTSEV HNWTLEDTLQ WLIEFVELPQ
241YEKNFRDNNV KGTTLPRIAV HEPSFMISQL KISDRSHRQK LQLKALDVVL FGPLTRPPHN
301WMKDFILTVS IVIGVGGCWF AYTQNKTSKE HVAKMMKDLE SLQTAEQSLM DLQERLEKAQ
361EENRNVAVEK QNLERKMMDE INYAKEEACR LRELREGAEC ELSRRQYAEQ ELEQVRMALK
421KAEKEFELRS SWSVPDALQK WLQLTHEVEV QYYNIKRQNA EMQLAIAKDE AEKIKKKRST
481VFGTLHVAHS SSLDEVDHKI LEAKKALSEL TTCLRERLFR WQQIEKICGF QIAHNSGLPS
541LTSSLYSDHS WVVMPRVSIP PYPIAGGVDD LDEDTPPIVS QFPGTMAKPP GSLARSSSLC
601RSRRSIVPSS PQPQRAQLAP HAPHPSHPRH PHHPQHTPHS LPSPDPDILS VSSCPALYRN
661EEEEEAIYFS AEKQWEVPDT ASECDSLNSS IGRKQSPPLS LEIYQTLSPR KISRDEVSLE
721DSSRGDSPVT VDVSWGSPDC VGLTETKSMI FSPASKVYNG ILEKSCSMNQ LSSGIPVPKP
781RHTSCSSAGN DSKPVQEAPS VARISSIPHD LCHNGEKSKK PSKIKSLFKK KSK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

833 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.