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STAU2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

STAU2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G113981-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human STAU2 (NM_014393), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
39K2 | 39K3 | Staufen homolog 2 | STAU2
Origin Species
Human
NCBI GenBank
NM_014393

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
52.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLQINQMFSV QLSLGEQTWE SEGSSIKKAQ QAVANKALTE STLPKPVQKP PKSNVNNNPG
61SITPTVELNG LAMKRGEPAI YRPLDPKPFP NYRANYNFRG MYNQRYHCPV PKIFYVQLTV
121GNNEFFGEGK TRQAARHNAA MKALQALQNE PIPERSPQNG ESGKDMDDDK DANKSEISLV
181FEIALKRNMP VSFEVIKESG PPHMKSFVTR VSVGEFSAEG EGNSKKLSKK RAATTVLQEL
241KKLPPLPVVE KPKLFFKKRP KTIVKAGPEY GQGMNPISRL AQIQQAKKEK EPDYVLLSER
301GMPRRREFVM QVKVGNEVAT GTGPNKKIAK KNAAEAMLLQ LGYKASTNLQ DQLEKTGENK
361GWSGPKPGFP EPTNNTPKGI LHLSPDVYQE MEASRHKVIS GTTLGYLSPK DMNQPSSSFF
421SISPTSNSSA TIARELLMNG TSSTAEAIGL KGSSPTPPCS PVQPSKQLEY LARIQGFQV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

479 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.