Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
STAU1 / Staufen Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G120502-100
Catalog Number: LS-G120502
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Staufen(STAU1) (NM_001037328), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- STAU1 | Staufen | STAU
- Origin Species
- Human
- NCBI GenBank
- NM_017453
- SwissProt
- O95793
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 55.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKLGKKPMYK PVDPYSRMQS TYNYNMRGGA YPPRYFYPFP VPPLLYQVEL SVGGQQFNGK61GKTRQAAKHD AAAKALRILQ NEPLPERLEV NGRESEEENL NKSEISQVFE IALKRNLPVN121FESFPLKQVA RESGPPHMKN FVTKVSVGEF VGEGEGKSKK ISKKNAAIAV LEELKKLPPL181PAVERVKPRI KKKTKPIVKP QTSPEYGQGI NPISRLAQIQ QAKKEKEPEY TLLTERGLPR241RREFVMQVKV GNHTAEGTGT NKKVAKRNAA ENMLEILGFK VPQAQPTKPA LKSEEKTPIK301KPGDGRKVTF FEPGSGDENG TSNKEDEFRM PYLSHQQLPA GILPMVPEVA QAVGVSQGHH361TKDFTRAAPN PAKATVTAMI ARELLYGGTS PTAETILKNN ISSGHVPHGP LTRPSEQLDY421LSRVQGFQVE YKDFPKNNKN EFVSLINCSS QPPLISHGIG KDVESCHDMA ALNILKLLSE481LDQQSTEMPR TGNGPMSVCG RCTRTRPLEQKLISEEDLAANDILDYKDDDDKV
502 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.