Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
STAU1 / Staufen Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G116542-100
Catalog Number: LS-G116542
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Staufen(STAU1) (NM_004602), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- STAU1 | Staufen | STAU
- Origin Species
- Human
- NCBI GenBank
- NM_017453
- SwissProt
- O95793
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 54.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKLGKKPMYK PVDPYSRMQS TYNYNMRGGA YPPRYFYPFP VPPLLYQVEL SVGGQQFNGK61GKTRQAAKHD AAAKALRILQ NEPLPERLEV NGRESEEENL NKSEISQVFE IALKRNLPVN121FEVARESGPP HMKNFVTKVS VGEFVGEGEG KSKKISKKNA AIAVLEELKK LPPLPAVERV181KPRIKKKTKP IVKPQTSPEY GQGINPISRL AQIQQAKKEK EPEYTLLTER GLPRRREFVM241QVKVGNHTAE GTGTNKKVAK RNAAENMLEI LGFKVPQAQP TKPALKSEEK TPIKKPGDGR301KVTFFEPGSG DENGTSNKED EFRMPYLSHQ QLPAGILPMV PEVAQAVGVS QGHHTKDFTR361AAPNPAKATV TAMIARELLY GGTSPTAETI LKNNISSGHV PHGPLTRPSE QLDYLSRVQG421FQVEYKDFPK NNKNEFVSLI NCSSQPPLIS HGIGKDVESC HDMAALNILK LLSELDQQST481EMPRTGNGPM SVCGRCTRTRPLEQKLISEEDLAANDILDYKDDDDKV
496 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.