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STARD8 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

STARD8 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all STARD8 antibodies →
$504.00each
Catalog Number: LS-G113763-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human STARD8 (NM_014725), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ARHGAP38 | DLC-3 | KIAA0189 | START domain containing 8 | START-GAP3 | STARD8 | DLC3 | STARTGAP3
Origin Species
Human
NCBI GenBank
NM_014725
SwissProt
Q92502

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
112.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTLNNCASMK LEVHFQSKQN EDSEEEEQCT ISSHWAFQQE SKCWSPMGSS DLLAPPSPGL
61PATSSCESVL TELSATSLPV ITVSLPPEPA DLPLPGRAPS SSDRPLLSPT QGQEGPQDKA
121KKRHRNRSFL KHLESLRRKE KSGSQQAEPK HSPATSEKVS KASSFRSCRG FLSAGFYRAK
181NWAATSAGGS GANTRKAWEA WPVASFRHPQ WTHRGDCLVH VPGDHKPGTF PRSLSIESLC
241PEDGHRLADW QPGRRWGCEG RRGSCGSTGS HASTYDNLPE LYPAEPVMVG AEAEDEDDEE
301SGGSYAHLDD ILQHVWGLQQ RVELWSRAMY PDLGPGDEEE EEATSSVEIA TVEVKCQAEA
361LSQMEVPAHG ESPAWAQAEV QPAVLAPAQA PAEAEPVAQE EAEAPAPAPA PAPAQDSEQE
421AHSGGEPTFA SSLSVEEGHS ISDTVASSSE LDSSGNSMNE AEAAGPLAGL QASMPRERRD
481SGVGASLTRP CRKLRWHSFQ NSHRPSLNSE SLEINRQFAG QINLLHKGSL LRLTAFMEKY
541TVPHKQGWVW SMPKFMRRNK TPDYRGQHVF GVPPLIHVQR TGQPLPQSIQ QAMRYLRSQC
601LDQVGIFRKS GVKSRIQNLR QMNETSPDNV CYEGQSAYDV ADLLKQYFRD LPEPIFTSKL
661TTTFLQIYQL LPKDQWLAAA QAATLLLPDE NREVLQTLLY FLSDIASAEE NQMTAGNLAV
721CLAPSIFHLN VSKKDSPSPR IKSKRSLIGR PGPRDLSDNM AATQGLSHMI SDCKKLFQVP
781QDMVLQLCSS YSAAELSPPG PALAELRQAQ AAGVSLSLYM EENIQDLLRD AAERFKGWMS
841VPGPQHTELA CRKAPDGHPL RLWKASTEVA APPAVVLHRV LRERALWDED LLRAQVLEAL
901MPGVELYHYV TDSMAPHPCR DFVVLRMWRS DLPRGGCLLV SQSLDPEQPV PESGVRALML
961TSQYLMEPCG LGRSRLTHIC RADLRGRSPD WYNKVFGHLC AMEVAKIRDS FPTLQAAGPE
1021TKL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1023 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.