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STARD13 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

STARD13 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G104788-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human STARD13 (NM_178006), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
46H23.2 | DLC-2 | DLC2 | GT650 | LINC00464 | Rho GTPase-activating protein | START domain containing 13 | ARHGAP37 | STARD13
Origin Species
Human
NCBI GenBank
NM_178006
SwissProt
Q9Y3M8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
124.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MFSQVPRTPA SGCYYLNSMT PEGQEMYLRF DQTTRRSPYR MSRILARHQL VTKIQQEIEA
61KEACDWLRAA GFPQYAQLYE DSQFPINIVA VKNDHDFLEK DLVEPLCRRL NTLNKCASMK
121LDVNFQRKKG DDSDEEDLCI SNKWTFQRTS RRWSRVDDLY TLLPRGDRNG SPGGTGMRNT
181TSSESVLTDL SEPEVCSIHS ESSGGSDSRS QPGQCCTDNP VMLDAPLVSS SLPQPPRDVL
241NHPFHPKNEK PTRARAKSFL KRMETLRGKG AHGRHKGSGR TGGLVISGPM LQQEPESFKA
301MQCIQIPNGD LQNSPPPACR KGLPCSGKSS GESSPSEHSS SGVSTPCLKE RKCHEANKRG
361GMYLEDLDVL AGTALPDAGD QSRMHEFHSQ ENLVVHIPKD HKPGTFPKAL SIESLSPTDS
421SNGVNWRTGS ISLGREQVPG AREPRLMASC HRASRVSIYD NVPGSHLYAS TGDLLDLEKD
481DLFPHLDDIL QHVNGLQEVV DDWSKDVLPE LQTHDTLVGE PGLSTFPSPN QITLDFEGNS
541VSEGRTTPSD VERDVTSLNE SEPPGVRDRR DSGVGASLTR PNRRLRWNSF QLSHQPRPAP
601ASPHISSQTA SQLSLLQRFS LLRLTAIMEK HSMSNKHGWT WSVPKFMKRM KVPDYKDKAV
661FGVPLIVHVQ RTGQPLPQSI QQALRYLRSN CLDQVGLFRK SGVKSRIHAL RQMNENFPEN
721VNYEDQSAYD VADMVKQFFR DLPEPLFTNK LSETFLHIYQ YVSKEQRLQA VQAAILLLAD
781ENREVLQTLL CFLNDVVNLV EENQMTPMNL AVCLAPSLFH LNLLKKESSP RVIQKKYATG
841KPDQKDLNEN LAAAQGLAHM IMECDRLFEV PHELVAQSRN SYVEAEIHVP TLEELGTQLE
901ESGATFHTYL NHLIQGLQKE AKEKFKGWVT CSSTDNTDLA FKKVGDGNPL KLWKASVEVE
961APPSVVLNRV LRERHLWDED FVQWKVVETL DRQTEIYQYV LNSMAPHPSR DFVVLRTWKT
1021DLPKGMCTLV SLSVEHEEAQ LLGGVRAVVM DSQYLIEPCG SGKSRLTHIC RIDLKGHSPE
1081WYSKGFGHLC AAEVARIRNS FQPLIAEGPE TKI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1113 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.