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STAG3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

STAG3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G114418-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human STAG3 (NM_012447), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
SCC3 homolog 3 | Stromal antigen 3 | Stromalin 3 | Stromalin-3 | Cohesin subunit SA-3 | STAG3
Origin Species
Human
NCBI GenBank
NM_012447
SwissProt
Q9UJ98

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
139 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSSPLQRAVG DTKRALSASS SSSASLPFDD RDSNHTSEGN GDSLLADEDT DFEDSLNRNV
61KKRAAKRPPK TTPVAKHPKK GSRVVHRHSR KQSEPPANDL FNAVKAAKSD MQSLVDEWLD
121SYKQDQDAGF LELVNFFIQS CGCKGIVTPE MFKKMSNSEI IQHLTEQFNE DSGDYPLIAP
181GPSWKKFQGS FCEFVRTLVC QCQYSLLYDG FPMDDLISLL TGLSDSQVRA FRHTSTLAAM
241KLMTSLVKVA LQLSVHQDNN QRQYEAERNK GPGQRAPERL ESLLEKRKEL QEHQEEIEGM
301MNALFRGVFV HRYRDVLPEI RAICIEEIGC WMQSYSTSFL TDSYLKYIGW TLHDKHREVR
361LKCVKALKGL YGNRDLTTRL ELFTSRFKDR MVSMVMDREY DVAVEAVRLL ILILKNMEGV
421LTDADCESVY PVVYASHRGL ASAAGEFLYW KLFYPECEIR MMGGREQRQS PGAQRTFFQL
481LLSFFVESEL HDHAAYLVDS LWDCAGARLK DWEGLTSLLL EKDQNLGDVQ ESTLIEILVS
541SARQASEGHP PVGRVTGRKG LTSKERKTQA DDRVKLTEHL IPLLPQLLAK FSADAEKVTP
601LLQLLSCFDL HIYCTGRLEK HLELFLQQLQ EVVVKHAEPA VLEAGAHALY LLCNPEFTFF
661SRADFARSQL VDLLTDRFQQ ELEELLQSSF LDEDEVYNLA ATLKRLSAFY NTHDLTRWEL
721YEPCCQLLQK AVDTGEVPHQ VILPALTLVY FSILWTLTHI SKSDASQKQL SSLRDRMVAF
781CELCQSCLSD VDTEIQEQAF VLLSDLLLIF SPQMIVGGRD FLRPLVFFPE ATLQSELASF
841LMDHVFIQPG DLGSGDSQED HLQIERLHQR RRLLAGFCKL LLYGVLEMDA ASDVFKHYNK
901FYNDYGDIIK ETLTRARQID RSHCSRILLL SLKQLYTELL QEHGPQGLNE LPAFIEMRDL
961ARRFALSFGP QQLQNRDLVV MLHKEGIQFS LSELPPAGSS NQPPNLAFLE LLSEFSPRLF
1021HQDKQLLLSY LEKCLQHVSQ APGHPWGPVT TYCHSLSPVE NTAETSPQVL PSSKRRRVEG
1081PAKPNREDVS SSQEESLQLN SIPPTPTLTS TAVKSRQPLW GLKEMEEEDG SELDFAQGQP
1141VAGTERSRFL GPQYFQTPHN PSGPGLGNQL MRLSLMEEDE EEELEIQDES NEERQDTDMQ
1201ASSYSSTSER GLDLLDSTEL DIEDF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1225 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.