Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
STAG1 / SA1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G115695-100
Catalog Number: LS-G115695
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SA1(STAG1) (NM_005862), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Cohesin subunit SA-1 | SA-1 | SCC3 homolog 1 | SCC3A | Stromal antigen 1 | SA1 | STAG1
- Origin Species
- Human
- NCBI GenBank
- NM_005862
- SwissProt
- Q8WVM7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 144.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MITSELPVLQ DSTNETTAHS DAGSELEETE VKGKRKRGRP GRPPSTNKKP RKSPGEKSRI61EAGIRGAGRG RANGHPQQNG EGEPVTLFEV VKLGKSAMQS VVDDWIESYK QDRDIALLDL121INFFIQCSGC RGTVRIEMFR NMQNAEIIRK MTEEFDEDSG DYPLTMPGPQ WKKFRSNFCE181FIGVLIRQCQ YSIIYDEYMM DTVISLLTGL SDSQVRAFRH TSTLAAMKLM TALVNVALNL241SIHQDNTQRQ YEAERNKMIG KRANERLELL LQKRKELQEN QDEIENMMNS IFKGIFVHRY301RDAIAEIRAI CIEEIGVWMK MYSDAFLNDS YLKYVGWTLH DRQGEVRLKC LKALQSLYTN361RELFPKLELF TNRFKDRIVS MTLDKEYDVA VEAIRLVTLI LHGSEEALSN EDCENVYHLV421YSAHRPVAVA AGEFLHKKLF SRHDPQAEEA LAKRRGRNSP NGNLIRMLVL FFLESELHEH481AAYLVDSLWE SSQELLKDWE CMTELLLEEP VQGEEAMSDR QESALIELMV CTIRQAAEAH541PPVGRGTGKR VLTAKERKTQ IDDRNKLTEH FIITLPMLLS KYSADAEKVA NLLQIPQYFD601LEIYSTGRME KHLDALLKQI KFVVEKHVES DVLEACSKTY SILCSEEYTI QNRVDIARSQ661LIDEFVDRFN HSVEDLLQEG EEADDDDIYN VLSTLKRLTS FHNAHDLTKW DLFGNCYRLL721KTGIEHGAMP EQIVVQALQC SHYSILWQLV KITDGSPSKE DLLVLRKTVK SFLAVCQQCL781SNVNTPVKEQ AFMLLCDLLM IFSHQLMTGG REGLQPLVFN PDTGLQSELL SFVMDHVFID841QDEENQSMEG DEEDEANKIE ALHKRRNLLA AFSKLIIYDI VDMHAAADIF KHYMKYYNDY901GDIIKETLSK TRQIDKIQCA KTLILSLQQL FNELVQEQGP NLDRTSAHVS GIKELARRFA961LTFGLDQIKT REAVATLHKD GIEFAFKYQN QKGQEYPPPN LAFLEVLSEF SSKLLRQDKK1021TVHSYLEKFL TEQMMERRED VWLPLISYRN SLVTGGEDDR MSVNSGSSSS KTSSVRNKKG1081RPPLHKKRVE DESLDNTWLN RTDTMIQTPG PLPAPQLTST VLRENSRPMG DQIQEPESEH1141GSEPDFLHNP QMQISWLGQP KLEDLNRKDR TGMNYMKVRT GVRHAVRGLM EEDAEPIFED1201VMMSSRSQLE DMNEEFEDTM VIDLPPSRNR RERAELRPDF FDSAAIIEDD SGFGMPMFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1258 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.