Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ST8SIA2 / STX Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G115587-100
Catalog Number: LS-G115587
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ST8SIA2 (NM_006011), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Alpha-2 | 8-sialyltransferase 8B | Sialyltransferase 8B | SIAT8B | ST8SIA-II | ST8SiaII | Sialyltransferase X | Sialytransferase St8Sia II | SIAT8-B | ST8SIA2 | STX | HsT19690
- Origin Species
- Human
- NCBI GenBank
- NM_006011
- SwissProt
- Q92186
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 42.2 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MQLQFRSWML AALTLLVVFL IFADISEIEE EIGNSGGRGT IRSAVNSLHS KSNRAEVVIN61GSSSPAVVDR SNESIKHNIQ PASSKWRHNQ TLSLRIRKQI LKFLDAEKDI SVLKGTLKPG121DIIHYIFDRD STMNVSQNLY ELLPRTSPLK NKHFGTCAIV GNSGVLLNSG CGQEIDAHSF181VIRCNLAPVQ EYARDVGLKT DLVTMNPSVI QRAFEDLVNA TWREKLLQRL HSLNGSILWI241PAFMARGGKE RVEWVNELIL KHHVNVRTAY PSLRLLHAVR GYWLTNKVHI KRPTTGLLMY301TLATRFCKQI YLYGFWPFPL DQNQNPVKYH YYDSLKYGYT SQASAHTMPL EFKALKSLHE361QGALKLTVGQ CDGATTRTRPLEQKLISEEDLAANDILDYKDDDDKV
375 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.