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ST7 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ST7 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G111744-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ST7 (NM_018412), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ETS7q | FAM4A | HELG | Protein FAM4A1 | RAY1 | SEN4 | ST7 | TSG7 | FAM4A1 | Protein HELG
Origin Species
Human
NCBI GenBank
NM_021908
SwissProt
Q9NRC1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
63.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAEAATGFLE QLKSCIVWSW TYLWTVWFFI VLFLVYILRV PLKINDNLST VSMFLNTLTP
61KFYVALTGTS SLISGLILIF EWWYFRKYGT SFIEQVSVSH LRPLLGGVDN NSSNNSNSSN
121GDSDSNRQSV SECKVWRNPL NLFRGAEYNR YTWVTGREPL TYYDMNLSAQ DHQTFFTCDS
181DHLRPADAIM QKAWRERNPQ ARISAAHEAL EINECATAYI LLAEEEATTI AEAEKLFKQA
241LKAGDGCYRR SQQLQHHGSQ YEAQHRRDTN VLVYIKRRLA MCARRLGRTR EAVKMMRDLM
301KEFPLLSMFN IHENLLEALL ELQAYADVQA VLAKYDDISL PKSATICYTA ALLKARAVSD
361KFSPEAASRR GLSTAEMNAV EAIHRAVEFN PHVPKYLLEM KSLILPPEHI LKRGDSEAIA
421YAFFHLAHWK RVEGALNLLH CTWEGTFRMI PYPLEKGHLF YPYPICTETA DRELLPSFHE
481VSVYPKKELP FFILFTAGLC SFTAMLALLT HQFPELMGVF AKAFLSTLFA PLNFVMEKVE
541SILPSSLWHQ LTRI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

554 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.