LSBio
ST6GALNAC1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ST6GALNAC1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ST6GALNAC1 antibodies →
$725.00each
Catalog Number: LS-G101563-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ST6GALNAC1 (NM_018414), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
SIAT7A | Sialyltransferase 7A | ST6GalNAcI | ST6GALNAC1 | STYI | HSY11339 | SIAT7-A | ST6GalNAc I
Origin Species
Human
NCBI GenBank
NM_018414

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
68.4 kda
Protein Type
Lysate
Amino Acid Sequence
1MRSCLWRCRH LSQGVQWSLL LAVLVFFLFA LPSFIKEPQT KPSRHQRTEN IKERSLQSLA
61KPKSQAPTRA RRTTIYAEPV PENNALNTQT QPKAHTTGDR GKEANQAPPE EQDKVPHTAQ
121RAAWKSPEKE KTMVNTLSPR GQDAGMASGR TEAQSWKSQD TKTTQGNGGQ TRKLTASRTV
181SEKHQGKAAT TAKTLIPKSQ HRMLAPTGAV STRTRQKGVT TAVIPPKEKK PQATPPPAPF
241QSPTTQRNQR LKAANFKSEP RWDFEEKYSF EIGGLQTTCP DSVKIKASKS LWLQKLFLPN
301LTLFLDSRHF NQSEWDRLEH FAPPFGFMEL NYSLVQKVVT RFPPVPQQQL LLASLPAGSL
361RCITCAVVGN GGILNNSHMG QEIDSHDYVF RLSGALIKGY EQDVGTRTSF YGFTAFSLTQ
421SLLILGNRGF KNVPLGKDVR YLHFLEGTRD YEWLEALLMN QTVMSKNLFW FRHRPQEAFR
481EALHMDRYLL LHPDFLRYMK NRFLRSKTLD GAHWRIYRPT TGALLLLTAL QLCDQVSAYG
541FITEGHERFS DHYYDTSWKR LIFYINHDFK LEREVWKRLH DEGIIRLYQR PGPGTAKAKN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

600 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.