Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ST3GAL5 / GM3 Synthase Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G100168-100
Catalog Number: LS-G100168
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ST3GAL5 (NM_003896), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Alpha 2 | 3-sialyltransferase V | Ganglioside GM3 synthase | GM3 synthase | SATI | Sialyltransferase 9 | SIATGM3S | ST3GAL5 | ST3Gal V | SIAT9 | ST3GalV
- Origin Species
- Human
- NCBI GenBank
- NM_003896
- SwissProt
- Q9UNP4
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 47.8 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MRTKAAGCAE RRPLQPRTEA AAAPAGRAMP SEYTYVKLRS DCSRPSLQWY TRAQSKMRRP61SLLLKDILKC TLLVFGVWIL YILKLNYTTE ECDMKKMHYV DPDRVKRAQK YAQQVLQKEC121RPKFAKTSMA LLFEHRYSVD LLPFVQKAPK DSEAESKYDP PFGFRKFSSK VQTLLELLPE181HDLPEHLKAK TCRRCVVIGS GGILHGLELG HTLNQFDVVI RLNSAPVEGY SEHVGNKTTI241RMTYPEGAPL SDLEYYSNDL FVAVLFKSVD FNWLQAMVKK ETLPFWVRLF FWKQVAEKIP301LQPKHFRILN PVIIKETAFD ILQYSEPQSR FWGRDKNVPT IGVIAVVLAT HLCDEVSLAG361FGYDLNQPRT PLHYFDSQCM AAMNFQTMHN VTTETKFLLK LVKEGVVKDL SGGIDREFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
418 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.