Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ST3GAL4 / ST3Gal IV Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G115419-100
Catalog Number: LS-G115419
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ST3GAL4 (NM_006278), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Alpha 2 | 3-ST 4 | CGS23 | Gal-NAc6S | NANTA3 | 3-sialyltransferase IV | SAT-3 | SAT3 | SIAT4-C | Sialyltransferase 4C | ST-4 | ST3GAL4 | ST3Gal IV | SIAT4 | SIAT4C | ST3GalA.2 | ST3GalIV | STZ
- Origin Species
- Human
- NCBI GenBank
- NM_006278
- SwissProt
- Q11206
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 37.3 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MVSKSRWKLL AMLALVLVVM VWYSISREDS FYFPIPEKKE PCLQGEAESK ASKLFGNYSR61DQPIFLRLED YFWVKTPSAY ELPYGTKGSE DLLLRVLAIT SSSIPKNIQS LRCRRCVVVG121NGHRLRNSSL GDAINKYDVV IRLNNAPVAG YEGDVGSKTT MRLFYPESAH FDPKVENNPD181TLLVLVAFKA MDFHWIETIL SDKKRVRKGF WKQPPLIWDV NPKQIRILNP FFMEIAADKL241LSLPMQQPRK IKQKPTTGLL AITLALHLCD LVHIAGFGYP DAYNKKQTIH YYEQITLKSM301AGSGHNVSQE ALAIKRMLEM GAIKNLTSFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
329 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.