Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ST3GAL3 / ST3N Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G105098-100
Catalog Number: LS-G105098
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ST3GAL3 (NM_174963), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Alpha 2 | 3-ST 3 | EIEE15 | MRT12 | Alpha-2 | 3-sialyltransferase II | SIAT6 | Sialyltransferase 6 | ST3GALII | ST3GalIII | ST3N | ST3Gal III | ST3GAL3
- Origin Species
- Human
- NCBI GenBank
- NM_006279
- SwissProt
- Q11203
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 49.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGLLVFVRNL LLALCLFLVL GFLYYSAWKL HLLQWEEDSS KYSHSSSPQE KPVADSVVLS61FDSAGQTLGS EYDRLGFLLN LDSKLSPRTL CTVVFGLDCI LESPGEPKKL LMPASHPLEI121LKSLSEDTAF ALGFLKLPRP AELATKYANF SEGACKPGYA SALMTAIFPR FSKPAPMFLD181DSFRKWARIR EFVPPFGIKG QDNLIKAILS VTKEYRLTPA LDSLRCRRCI IVGNGGVLAN241KSLGSRIDDY DIVVRLNSAP VKGFEKDVGS KTTLRITYPE GAMQRPEQYE RDSLFVLAGF301KWQDFKWLKY IVYKERVSAS DGFWKSVATR VPKEPPEIRI LNPYFIQEAA FTLIGLPFNN361GLMGRGNIPT LGSVAVTMAL HGCDEVAVAG FGYDMSTPNA PLHYYETVRM AAIKESWTHN421IQREKEFLRK LVKARVITDL SSGITRTRPLEQKLISEEDLAANDILDYKDDDDKV
444 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.