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ST3GAL2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ST3GAL2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G114983-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ST3GAL2 (NM_006927), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Gal-NAc6S | Alpha 2 | 3-ST 2 | ST3GALII | ST3GAL2 | SIAT4B | ST3GalA.2 | Sialyltransferase 4B | SIAT4-B | ST3Gal II
Origin Species
Human
NCBI GenBank
NM_006927
SwissProt
Q16842

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
40 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKCSLRVWFL SVAFLLVFIM SLLFTYSHHS MATLPYLDSG ALDGTHRVKL VPGYAGLQRL
61SKERLSGKSC ACRRCMGDAG ASDWFDSHFD GNISPVWTRE NMDLPPDVQR WWMMLQPQFK
121SHNTNEVLEK LFQIVPGENP YRFRDPHQCR RCAVVGNSGN LRGSGYGQDV DGHNFIMRMN
181QAPTVGFEQD VGSRTTHHFM YPESAKNLPA NVSFVLVPFK VLDLLWIASA LSTGQIRFTY
241APVKSFLRVD KEKVQIYNPA FFKYIHDRWT EHHGRYPSTG MLVLFFALHV CDEVNVYGFG
301ADSRGNWHHY WENNRYAGEF RKTGVHDADF EAHIIDMLAK ASKIEVYRGN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

350 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.