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ST18 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ST18 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ST18 antibodies →
$725.00each
Catalog Number: LS-G113782-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ST18 (NM_014682), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0535 | Zinc finger protein 387 | ST18 | ZC2HC10 | ZNF387
Origin Species
Human
NCBI GenBank
NM_014682
SwissProt
O60284

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
115 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDAEAEDKTL RTRSKGTEVP MDSLIQELSV AYDCSMAKKR TAEDQALGVP VNKRKSLLMK
61PRHYSPKADC QEDRSDRTED DGPLETHGHS TAEEIMIKPM DESLLSTAQE NSSRKEDRYS
121CYQELMVKSL MHLGKFEKNV SVQTVSENLN DSGIQSLKAE SDEADECFLI HSDDGRDKID
181DSQPPFCSSD DNESNSESAE NGWDSGSNFS EETKPPRVPK YVLTDHKKDL LEVPEIKTEG
241DKFIPCENRC DSETERKDPQ NALAEPLDGN AQPSFPDVEE EDSESLAVMT EEGSDLEKAK
301GNLSLLEQAI ALQAERGCVF HNTYKELDRF LLEHLAGERR QTKVIDMGGR QIFNNKHSPR
361PEKRETKCPI PGCDGTGHVT GLYPHHRSLS GCPHKVRVPL EILAMHENVL KCPTPGCTGR
421GHVNSNRNTH RSLSGCPIAA AEKLAMSQDK NQLDSPQTGQ CPDQAHRTSL VKQIEFNFPS
481QAITSPRATV SKEQEKFGKV PFDYASFDAQ VFGKRPLIQT VQGRKTPPFP ESKHFPNPVK
541FPNRLPSAGA HTQSPGRASS YSYGQCSEDT HIAAAAAILN LSTRCREATD ILSNKPQSLH
601AKGAEIEVDE NGTLDLSMKK NRILDKSAPL TSSNTSIPTP SSSPFKTSSI LVNAAFYQAL
661CDQEGWDTPI NYSKTHGKTE EEKEKDPVSS LENLEEKKFP GEASIPSPKP KLHARDLKKE
721LITCPTPGCD GSGHVTGNYA SHRSVSGCPL ADKTLKSLMA ANSQELKCPT PGCDGSGHVT
781GNYASHRSLS GCPRARKGGV KMTPTKEEKE DPELKCPVIG CDGQGHISGK YTSHRTASGC
841PLAAKRQKEN PLNGASLSWK LNKQELPHCP LPGCNGLGHV NNVFVTHRSL SGCPLNAQVI
901KKGKVSEELM TIKLKATGGI ESDEEIRHLD EEIKELNESN LKIEADMMKL QTQITSMESN
961LKTIEEENKL IEQNNESLLK ELAGLSQALI SSLADIQLPQ MGPISEQNFE AYVNTLTDMY
1021SNLERDYSPE CKALLESIKQ AVKGIHV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1047 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.