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SSX2IP Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SSX2IP Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all SSX2IP antibodies →
$504.00each
Catalog Number: LS-G114189-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SSX2IP (NM_014021), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ADIP | KIAA0923 | SSX2-interacting protein | SSX2IP
Origin Species
Human
NCBI GenBank
NM_014021

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
71.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGDWMTVTDP GLSSESKTIS QYTSETKMSP SSLYSQQVLC SSIPLSKNVH SFFSAFCTED
61NIEQSISYLD QELTTFGFPS LYEESKGKET KRELNIVAVL NCMNELLVLQ RKNLLAQENV
121ETQNLKLGSD MDHLQSCYSK LKEQLETSRR EMIGLQERDR QLQCKNRNLH QLLKNEKDEV
181QKLQNIIASR ATQYNHDMKR KEREYNKLKE RLHQLVMNKK DKKIAMDILN YVGRADGKRG
241SWRTGKTEAR NEDEMYKILL NDYEYRQKQI LMENAELKKV LQQMKKEMIS LLSPQKKKPR
301ERVDDSTGTV ISDVEEDAGE LSRESMWDLS CETVREQLTN SIRKQWRILK SHVEKLDNQV
361SKVHLEGFND EDVISRQDHE QETEKLELEI QQCKEMIKTQ QQLLQQQLAT AYDDDTTSLL
421RDCYLLEEKE RLKEEWSLFK EQKKNFERER RSFTEAAIRL GLERKAFEEE RASWLKQQFL
481NMTTFDHQNS ENVKLFSAFS GSSDWDNLIV HSRQPQKKPH SVSNGSPVCM SKLTKSLPAS
541PSTSDFCQTR SCISEHSSIN VLNITAEEIK PNQVGGECTN QKWSVASRPG SQEGCYSGCS
601LSYTNSHVEK DDLP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

614 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.