
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SSUH2 / C3orf32 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G112956-100
Catalog Number: LS-G112956
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C3orf32(SSUH2) (NM_015931), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Fls485 | SSUH2 | Ssu-2 homolog (C. elegans) | SSU-2 | C3orf32
- Origin Species
- Human
- NCBI GenBank
- NM_015931
- SwissProt
- Q9Y2M2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 39.7 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MPSPVGLLRA LPLPWPQFLA CTLRRLAGPR ESTGPSQKPP PLCSVPCRVP AMTEEVAREA61LLSFVDSKCC YSSTVAGDLV IQELKRQTLC RYRLETFSES RISEWTFQPF TNHSVDGPQR121GASPRLWDIK VQGPPMFQED TRKFQVPHSS LVKECHKCHG RGRYKCSGCH GAGTVRCPSC181CGAKRKAKQS RRCQLCAGSG RRRCSTCSGR GNKTCATCKG EKKLLHFIQL VIMWKNSLFE241FVSEHRLNCP RELLAKAKGE NLFKDENSVV YPIVDFPLRD ISLASQRGIA EHSAALASRA301RVLQQRQTIE LIPLTEVHYW YQGKTYVYYI YGTDHQVYAV DYPERYCCGC TIVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
353 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.
