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SRRM3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SRRM3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G124189-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SRRM3 (NM_001110199), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
SRRM3 | SRRM2-like protein
Origin Species
Human
NCBI GenBank
NM_001110199
SwissProt
A6NNA2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
71.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSSTVNNGAA SMQSTPDAAN GFPQPSSSSG TWPRAEEELR AAEPGLVKRA HREILDHERK
61RRVELKCMEL QEMMEEQGYS EEEIRQKVGT FRQMLMEKEG VLTREDRPGG HIVAETPRLT
121EGAEPGLEYA PFDDDDGPVD CDCPASCYRG HRGYRTKHWS SSSASPPPKK KKKKKGGHRR
181SRKKRRLESE CSCGSSSPLR KKKKSVKKHR RDRSDSGSRR KRRHRSRSSK CKRKEKNKEK
241KRPHTESPGR RSHRHSSGSS HSPSLSSHYS DSRSPSRLSP KHRDEGRKTG SQRSSGSRSP
301SPSGGSGWGS PQRNGGSGQR SGAHGGRPGS AHSPPDKPSS PSPRVRDKAA AAAPTPPARG
361KESPSPRSAP SSQGRGGRAA GGAGRRRRRR RRRRRSRSSA SAPRRRGRRR PRPAPPRGSS
421RSLSRARSSS DSGSGRGAPG PGPEPGSERG HGGHGKRAKE RPPRARPAST SPSPGAHGRR
481GGPEGKSSSR SPGPHPRSWS SSRSPSKSRS RSAEKRPHSP SRSPSPKKPL SRDKDGEGRA
541RHSEAEATRA RRRSRSYSPI RKRRRDSPSF MEPRRITSAR KRPIPYYRPS PSSSSSCLSS
601DYSTRSHSRS PSPGHSHGSY SSRSHGTRSR TRSPSRTPSP SYHSRSSSES GGF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

653 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.