LSBio
SRPX Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SRPX Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all SRPX antibodies →
$504.00each
Catalog Number: LS-G129750-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SRPX (NM_001170751), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DRS | ETX1 | SRPX1 | SRPX
Origin Species
Human
NCBI GenBank
NM_006307
SwissProt
P78539

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
45.6 kda
Protein Type
Lysate
Amino Acid Sequence
1MGSPAHRPAL LLLLPPLLLL LLLRVPPSRS FPGSGDSPLE DDEVGYSHPR YKDTPWCSPI
61KVKYGDVYCR APQGGYYKTA LGTRCDIRCQ KGYELHGSSL LICQSNKRWS DKVICKHMEP
121PRIKCPSVKE RIAEPNKLTV RVSWETPEGR DTADGILTDV ILKGLPPGSN FPEGDHKIQY
181TVYDRAENKG TCKFRVKVRV KRCGKLNAPE NGYMKCSSDG DNYGATCEFS CIGGYELQGS
241PARVCQSNLA WSGTEPTCAA MNVNVGVRTA AALLDQFYEK RRLLIVSTPT ARNLLYRLQL
301GMLQQAQCGL DLRHITVVEL VGVFPTLIGR IGAKIMPPAL ALQLRLLLRI PLYSFSMVLV
361DKHGMDKERY VSLVMPVALF NLIDTFPLRK EEMVLQAEMS QTCNT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

405 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.