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SRPK2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SRPK2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G104175-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SRPK2 (NM_182692), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
H_RG152G17.1a | H_RG152G17.1b | SFRS protein kinase 2 | SFRSK2 | SRSF protein kinase 2 | SR-protein-specific kinase 2 | SRPK2 | MSRPK2 | SR protein kinase 2 | WUGSC:H_RG152G17.1a | Serine kinase SRPK2
Origin Species
Human
NCBI GenBank
U88666
SwissProt
P78362

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
78.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSSRKVLAIQ ARKRRPKREK HPKKPEPQQK APLVPPPPPP PPPPPPPLPD PTPPEPEEEI
61LGSDDEEQED PADYCKGGYH PVKIGDLFNG RYHVIRKLGW GHFSTVWLCW DMQGKRFVAM
121KVVKSAQHYT ETALDEIKLL KCVRESDPSD PNKDMVVQLI DDFKISGMNG IHVCMVFEVL
181GHHLLKWIIK SNYQGLPVRC VKSIIRQVLQ GLDYLHSKCK IIHTDIKPEN ILMCVDDAYV
241RRMAAEATEW QKAGAPPPSG SAVSTAPQQK PIGKISKNKK KKLKKKQKRQ AELLEKRLQE
301IEELEREAER KIIEENITSA APSNDQDGEY CPEVKLKTTG LEEAAEAETA KDNGEAEDQE
361EKEDAEKENI EKDEDDVDQE LANIDPTWIE SPKTNGHIEN GPFSLEQQLD DEDDDEEDCP
421NPEEYNLDEP NAESDYTYSS SYEQFNGELP NGRHKIPESQ FPEFSTSLFS GSLEPVACGS
481VLSEGSPLTE QEESSPSHDR SRTVSASSTG DLPKAKTRAA DLLVNPLDPR NADKIRVKIA
541DLGNACWVHK HFTEDIQTRQ YRSIEVLIGA GYSTPADIWS TACMAFELAT GDYLFEPHSG
601EDYSRDEDHI AHIIELLGSI PRHFALSGKY SREFFNRRGE LRHITKLKPW SLFDVLVEKY
661GWPHEDAAQF TDFLIPMLEM VPEKRASAGE CLRHPWLNS
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

699 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.