Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SRL / Sarcalumenin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G118988-100
Catalog Number: LS-G118988
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Sarcalumenin(SRL) (NM_001098814), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Sarcalumenin | SRL
- Origin Species
- Human
- NCBI GenBank
- NM_001098814
- SwissProt
- Q86TD4
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 54.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MRALVLLGCL LASLLFSGQA EETEDANEEA PLRDRSHIEK TLMLNEDKPS DDYSAVLQRL61RKIYHSSIKP LEQSYKYNEL RQHEITDGEI TSKPMVLFLG PWSVGKSTMI NYLLGLENTR121YQLYTGAEPT TSEFTVLMHG PKLKTIEGIV MAADSARSFS PLEKFGQNFL EKLIGIEVPH181KLLERVTFVD TPGIIENRKQ QERGYPFNDV CQWFIDRADL IFVVFDPTKL DVGLELEMLF241RQLKGRESQI RIILNKADNL ATQMLMRVYG ALFWSLAPLI NVTEPPRVYV SSFWPQEYKP301DTHQELFLQE EISLLEDLNQ VIENRLENKI AFIRQHAIRV RIHALLVDRY LQTYKDKMTF361FSDGELVFKD IVEDPDKFYI FKTILAKTNV SKFDLPNREA YKDFFGINPI SSFKLLSQQC421SYMGGCFLEK IERAITQELP GLLGSLGLGK NPGALNCDKT GCSETPKNRY RKHTRTRPLEQKLISEEDLAANDILDYKDDDDKV
473 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.