Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SREK1 / SRRP86 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G119953-100
Catalog Number: LS-G119953
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SFRS12(SREK1) (NM_001077199), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- SFRS12 | SREK1 | SRrp508 | SRrp86
- Origin Species
- Human
- NCBI GenBank
- NM_139168
- SwissProt
- Q8WXA9
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 71.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MNSGGGFGLG LGFGLTPTSV IQVTNLSSAV TSEQMRTLFS FLGEIEELRL YPPDNAPLAF61SSKVCYVKFR DPSSVGVAQH LTNTVFIDRA LIVVPCAEGK IPEESKALSL LAPAPTMTSL121MPGAGLLPIP TPNPLTTLGV SLSSLGAIPA AALDPNIATL GEIPQPPLMG NVDPSKIDEI181RRTVYVGNLN SQTTTADQLL EFFKQVGEVK FVRMAGDETQ PTRFAFVEFA DQNSVPRALA241FNGVMFGDRP LKINHSNNAI VKPPEMTPQA AAKELEEVMK RVREAQSFIS AAIEPESGKS301NERKGGRSRS HTRSKSRSSS KSHSRRKRSQ SKHRSRSHNR SRSRQKDRRR SKSPHKKRSK361SRERRKSRSR SHSRDKRKDT REKIKEKERV KEKDREKERE REKEREKEKE RGKNKDRDKE421REKDREKDKE KDREREREKE HEKDRDKEKE KEQDKEKERE KDRSKEIDEK RKKDKKSRTP481PRSYNASRRS RSSSRERRRR RSRSSSRSPR TSKTIKRKSS RSPSPRSRNK KDKKREKERD541HISERRERER STSMRKSSND RDGKEKLEKN STSLKEKEHN KEPDSSVSKE VDDKDAPRTE601ENKIQHNGNC QLNEENLSTK TEAVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
624 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.