Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SREK1 / SRRP86 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G107081-100
Catalog Number: LS-G107081
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SFRS12(SREK1) (NM_139168), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- SFRS12 | SREK1 | SRrp508 | SRrp86
- Origin Species
- Human
- NCBI GenBank
- NM_139168
- SwissProt
- Q8WXA9
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 59.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTSLMPGAGL LPIPTPNPLT TLGVSLSSLG AIPAAALDPN IATLGEIPQP PLMGNVDPSK61IDEIRRTVYV GNLNSQTTTA DQLLEFFKQV GEVKFVRMAG DETQPTRFAF VEFADQNSVP121RALAFNGVMF GDRPLKINHS NNAIVKPPEM TPQAAAKELE EVMKRVREAQ SFISAAIEPE181SGKSNERKGG RSRSHTRSKS RSSSKSHSRR KRSQSKHRSR SHNRSRSRQK DRRRSKSPHK241KRSKSRERRK SRSRSHSRDK RKDTREKIKE KERVKEKDRE KEREREKERE KEKERGKNKD301RDKEREKDRE KDKEKDRERE REKEHEKDRD KEKEKEQDKE KEREKDRSKE IDEKRKKDKK361SRTPPRSYNA SRRSRSSSRE RRRRRSRSSS RSPRTSKTIK RKSSRSPSPR SRNKKDKKRE421KERDHISERR ERERSTSMRK SSNDRDGKEK LEKNSTSLKE KEHNKEPDSS VSKEVDDKDA481PRTEENKIQH NGNCQLNEEN LSTKTEAVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
508 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.