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SRBD1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SRBD1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G111996-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SRBD1 (NM_018079), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
H_NH0576F01.1 | S1 RNA binding domain 1 | SRBD1 | WUGSC:H_NH0576F01.1
Origin Species
Human
NCBI GenBank
NM_018079
SwissProt
Q8N5C6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
111.6 kda
Protein Type
Lysate
Amino Acid Sequence
1MSSLPRRAKV QVQDVVLKDE FSSFSELSSA SEEDDKEDSA WEPQKKVPRS RKQPPPKESK
61PKRMPRVKKN APQISDGSEV VVVKEELNSS VAIADTALED RKNKLDTVQT LKTAKTKQKC
121AAQPHTVRRT KKLKVEEETS KASNLEGESN SSETPSTSTV WGGTCKKEEN DDDFTFGQSA
181LKKIKTETYP QGQPVKFPAN ANSTKEEVEM NWDMVQVLSE RTNIEPWVCA NIIRLFNDDN
241TIPFIIRYRK ELINNLDADS LREVQQTLEE LRAVAKKVHS TIQKIKKEGK MSECLLKAML
301NCKTFEELEH VSAPYKTGSK GTKAQRARQL GLEGAARALL EKPGELSLLS YIRPDVKGLS
361TLQDIEIGVQ HILADMIAKD KDTLDFIRNL CQKRHVCIQS SLAKVSSKKV NEKDVDKFLL
421YQHFSCNIRN IHHHQILAIN RGENLKVLTV KVNISDGVKD EFCRWCIQNR WRPRSFARPE
481LMKILYNSLN DSFKRLIYPL LCREFRAKLT SDAEKESVMM FGRNLRQLLL TSPVPGRTLM
541GVDPGYKHGC KLAIISPTSQ ILHTDVVYLH CGQGFREAEK IKTLLLNFNC STVVIGNGTA
601CRETEAYFAD LIMKNYFAPL DVVYCIVSEA GASIYSVGPE ANKEMPGLDP NLRSAVSIAR
661RVQDPLAELV KIEPKHIGVG MYQHDVSQTL LKATLDSVVE ECVSFVGVDI NICSEVLLRH
721IAGLNANRAK NIIEWREKNG PFINREQLKK VKGLGPKSFQ QCAGFIRINQ DYIRTFCSQQ
781TETSGQIQGV AVTSSADVEV TNEKQGKKKS KTAVNVLLKP NPLDQTCIHP ESYDIAMRFL
841SSIGGTLYEV GKPEMQQKIN SFLEKEGMEK IAERLQTTVH TLQVIIDGLS QPESFDFRTD
901FDKPDFKRSI VCLEDLQIGT VLTGKVENAT LFGIFVDIGV GKSGLIPIRN VTEAKLSKTK
961KRRSLGLGPG ERVEVQVLNI DIPRSRITLD LIRVL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

995 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.