Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SR140 / U2SURP Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G120202-100
Catalog Number: LS-G120202
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SR140(U2SURP) (NM_001080415), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- FSAPa | U2-associated protein SR140 | U2-associated SR140 protein | KIAA0332 | SR140 | U2 associated SR140 protein | U2SURP
- Origin Species
- Human
- NCBI GenBank
- AB002330
- SwissProt
- O15042
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 118.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MADKTPGGSQ KASSKTRSSD VHSSGSSDAH MDASGPSDSD MPSRTRPKSP RKHNYRNESA61RESLCDSPHQ NLSRPLLENK LKAFSIGKMS TAKRTLSKKE QEELKKKEDE KAAAEIYEEF121LAAFEGSDGN KVKTFVRGGV VNAAKEEHET DEKRGKIYKP SSRFADQKNP PNQSSNERPP181SLLVIETKKP PLKKGEKEKK KSNLELFKEE LKQIQEERDE RHKTKGRLSR FEPPQSDSDG241QRRSMDAPSR RNRSSGVLDD YAPGSHDVGD PSTTNLYLGN INPQMNEEML CQEFGRFGPL301ASVKIMWPRT DEERARERNC GFVAFMNRRD AERALKNLNG KMIMSFEMKL GWGKAVPIPP361HPIYIPPSMM EHTLPPPPSG LPFNAQPRER LKNPNAPMLP PPKNKEDFEK TLSQAIVKVV421IPTERNLLAL IHRMIEFVVR EGPMFEAMIM NREINNPMFR FLFENQTPAH VYYRWKLYSI481LQGDSPTKWR TEDFRMFKNG SFWRPPPLNP YLHGMSEEQE TEAFVEEPSK KGALKEEQRD541KLEEILRGLT PRKNDIGDAM VFCLNNAEAA EEIVDCITES LSILKTPLPK KIARLYLVSD601VLYNSSAKVA NASYYRKFFE TKLCQIFSDL NATYRTIQGH LQSENFKQRV MTCFRAWEDW661AIYPEPFLIK LQNIFLGLVN IIEEKETEDV PDDLDGAPIE EELDGAPLED VDGIPIDATP721IDDLDGVPIK SLDDDLDGVP LDATEDSKKN EPIFKVAPSK WEAVDESELE AQAVTTSKWE781LFDQHEESEE EENQNQEEES EDEEDTQSSK SEEHHLYSNP IKEEMTESKF SKYSEMSEEK841RAKLREIELK VMKFQDELES GKRPKKPGQS FQEQVEHYRD KLLQREKEKE LERERERDKK901DKEKLESRSK DKKEKDECTP TRKERKRRHS TSPSPSRSSS GRRVKSPSPK SERSERSERS961HKESSRSRSS HKDSPRDVSK KAKRSPSGSR TPKRSRRSRS RSPKKSGKKS RSQSRSPHRS1021HKKSKKNKHTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1029 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.