LSBio
SPTY2D1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SPTY2D1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all SPTY2D1 antibodies →
$725.00each
Catalog Number: LS-G103888-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SPTY2D1 (NM_194285), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_194285

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
75.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDFREILMIA SKGQGVNNVP KRYSLAVGPP KKDPKVKGVQ SAAVQAFLKR KEEELRRKAL
61EEKRRKEELV KKRIELKHDK KARAMAKRTK DNFHGYNGIP IEEKSKKRQA TESHTSQGTD
121REYEMEEENE FLEYNHAESE QEYEEEQEPP KVESKPKVPL KSAPPPMNFT DLLRLAEKKQ
181FEPVEIKVVK KSEERPMTAE ELREREFLER KHRRKKLETD GKLPPTVSKK APSQKESVGT
241KLSKGSGDRH PSSKGMPLPH AEKKSRPSMA NEKHLALSSS KSMPGERIKA GSGNSSQPSL
301REGHDKPVFN GAGKPHSSTS SPSVPKTSAS RTQKSAVEHK AKKSLSHPSH SRPGPMVTPH
361NKAKSPGVRQ PGSSSSSAPG QPSTGVARPT VSSGPVPRRQ NGSSSSGPER SISGSKKPTN
421DSNPSRRTVS GTCGPGQPAS SSGGPGRPIS GSVSSARPLG SSRGPGRPVS SPHELRRPVS
481GLGPPGRSVS GPGRSISGSI PAGRTVSNSV PGRPVSSLGP GQTVSSSGPT IKPKCTVVSE
541TISSKNIISR SSNGQMNGMK PPLSGYRAAQ GPQRLPFPTG YKRQREYEEE DDDDDEYDSE
601MEDFIEDEGE PQEEISKHIR EIFGYDRKKY KDESDYALRY MESSWKEQQK EEAKSLRLGM
661QEDLEEMRRE EEEMQRRRAK KLKRR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

685 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.