Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SPT5 / SUPT5H Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G125120-100
Catalog Number: LS-G125120
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SUPT5H (NM_001130825), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- SPT5 | SPT5H | Tat-CT1 protein | Tat-cotransactivator 1 protein | DSIF large subunit | DSIF p160 | HSPT5 | SUPT5H | Tat-CT1
- Origin Species
- Human
- NCBI GenBank
- NM_003169
- SwissProt
- O00267
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 120.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSDSEDSNFS EEEDSERSSD GEEAEVDEER RSAAGSEKEE EPEDEEEEEE EEEYDEEEEE61EDDDRPPKKP RHGGFILDEA DVDDEYEDED QWEDGAEDIL EKASNIDNVV LDEDRSGARR121LQNLWRDQRE EELGEYYMKK YAKSSVGETV YGGSDELSDD ITQQQLLPGV KDPNLWTVKC181KIGEERATAI SLMRKFIAYQ FTDTPLQIKS VVAPEHVKGY IYVEAYKQTH VKQAIEGVGN241LRLGYWNQQM VPIKEMTDVL KVVKEVANLK PKSWVRLKRG IYKDDIAQVD YVEPSQNTIS301LKMIPRIDYD RIKARMSLKD WFAKRKKFKR PPQRLFDAEK IRSLGGDVAS DGDFLIFEGN361RYSRKGFLFK SFAMSAVITE GVKPTLSELE KFEDQPEGID LEVVTESTGK EREHNFQPGD421NVEVCEGELI NLQGKILSVD GNKITIMPKH EDLKDMLEFP AQELRKYFKM GDHVKVIAGR481FEGDTGLIVR VEENFVILFS DLTMHELKVL PRDLQLCSET ASGVDVGGQH EWGELVQLDP541QTVGVIVRLE RETFQVLNMY GKVVTVRHQA VTRKKDNRFA VALDSEQNNI HVKDIVKVID601GPHSGREGEI RHLFRSFAFL HCKKLVENGG MFVCKTRHLV LAGGSKPRDV TNFTVGGFAP661MSPRISSPMH PSAGGQRGGF GSPGGGSGGM SRGRGRRDNE LIGQTVRISQ GPYKGYIGVV721KDATESTARV ELHSTCQTIS VDRQRLTTVG SRRPGGMTST YGRTPMYGSQ TPMYGSGSRT781PMYGSQTPLQ DGSRTPHYGS QTPLHDGSRT PAQSGAWDPN NPNTPSRAEE EYEYAFDDEP841TPSPQAYGGT PNPQTPGYPD PSSPQVNPQY NPQTPGTPAM YNTDQFSPYA APSPQGSYQP901SPSPQSYHQV APSPAGYQNT HSPASYHPTP SPMAYQASPS PSPVGYSPMT PGAPSPGGYN961PHTPGSGIEQ NSSDWVTTDI QVKVRDTYLD TQVVGQTGVI RSVTGGMCSV YLKDSEKVVS1021ISSEHLEPIT PTKNNKVKVI LGEDREATGV LLSIDGEDGI VRMDLDEQLK ILNLRFLGKL1081LEATRTRPLEQKLISEEDLAANDILDYKDDDDKV
1083 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.