Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SPT3 / SUPT3H Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G104496-100
Catalog Number: LS-G104496
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SPT3(SUPT3H) (NM_181356), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- SPT3 | SUPT3H | SPT3-like protein | SPT3L
- Origin Species
- Human
- NCBI GenBank
- NM_003599
- SwissProt
- O75486
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 37.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MVVFGTTFFN FDSFIGKTDS IEIIGKSVFP YCYHNILPLL NDSGRYSLGD ARRPLHETAV61LVEDVVHTQL INLLQQAAEV SQLRGARVIT PEDLLFLMRK DKKKLRRLLK YMFIRDYKSK121IVKGIDEDDL LEDKLSGSNN ANKRQKIAQD FLNSIDQTGE LLAMFEDDEI DEVKQERMER181AERQTRIMDS AQYAEFCESR QLSFSKKASK FRDWLDCSSM EIKPNVVAME ILAYLAYETV241AQLVDLALLV RQDMVTKAGD PFSHAISATF IQYHNSAEST AACGVEAHSD AIQPCHIREA301IRRYSHRIGP LSPFTNAYRR NGMAFLACTRTRPLEQKLISEEDLAANDILDYKDDDDKV
328 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.