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SPRR1A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SPRR1A Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G100697-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SPRR1A (NM_005987), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
19 kDa pancornulin | Cornifin-A | Small proline-rich protein 1A | SPRK | SPR-IA | Small proline-rich protein IA | Cornifin A | SPRR1A
Origin Species
Human
NCBI GenBank
NM_005987
SwissProt
P35321

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
9.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNSQQQKQPC TPPPQPQQQQ VKQPCQPPPQ EPCIPKTKEP CQPKVPEPCH PKVPEPCQPK
61IPEPCQPKVP EPCPSTVTPA PAQQKTKQK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

89 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.