Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SPP1 / Osteopontin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G99308-100
Catalog Number: LS-G99308
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Osteopontin(SPP1) (NM_001040060), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BSPI | BNSP | Bone sialoprotein 1 | ETA-1 | Nephropontin | OPN | Secreted phosphoprotein 1 | SPP-1 | SPP1/CALPHA1 fusion | Urinary stone protein | Uropontin | Osteopontin | Bone sialoprotein I | SPP1
- NCBI GenBank
- NM_000582
- SwissProt
- P10451
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 32.36 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MRIAVICFCL LGITCAIPVK QADSGSSEEK QNAVSSEETN DFKQETLPSK SNESHDHMDD61MDDEDDDDHV DSQDSIDSND SDDVDDTDDS HQSDESHHSD ESDELVTDFP TDLPATEVFT121PVVPTVDTYD GRGDSVVYGL RSKSKKFRRP DIQYPDATDE DITSHMESEE LNGAYKAIPV181AQDLNAPSDW DSRGKDSYET SQLDDQSAET HSHKQSRLYK RKANDESNEH SDVIDSQELS241KVSREFHSHE FHSHEDMLVV DPKSKEEDKH LKFRISHELD SASSEVNTRTRPLEQKLISEEDLAANDILDYKDDDDKV
287 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.