Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SPNS1 / Spinster Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G125939-100
Catalog Number: LS-G125939
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SPNS1 (NM_001142448), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HSpin1 | PP2030 | SPINL | Nrs | Protein spinster homolog 1 | Spinster-like protein 1 | SPNS1
- Origin Species
- Human
- SwissProt
- Q9H2V7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 56.4 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MAGSDTAPFL SQADDPDDGP VPGTPGLPGS TGNPKSEEPE VPDQEGLQRI TGLSPGRSAL61IVAVLCYINL LNYMDRFTVA GVLPDIEQFF NIGDSSSGLI QTVFISSYMV LAPVFGYLGD121RYNRKYLMCG GIAFWSLVTL GSSFIPGEHF WLLLLTRGLV GVGEASYSTI APTLIADLFV181ADQRSRMLSI FYFAIPVGSG LGYIAGSKVK DMAGDWHWAL RVTPGLGVVA VLLLFLVVRE241PPRGAVERHS DLPPLNPTSW WADLRALARN PSFVLSSLGF TAVAFVTGSL ALWAPAFLLR301SRVVLGETPP CLPGDSCSSS DSLIFGLITC LTGVLGVGLG VEISRRLRHS NPRADPLVCA361TGLLGSAPFL FLSLACARGS IVATYIFIFI GETLLSMNWA IVADILLYVV IPTRRSTAEA421FQIVLSHLLG DAGSPYLIGL ISDRLRRNWP PSFLSEFRAL QFSLMLCAFV GALGGAAFLG481TAIFIEADRR RAQLHVQGLL HEAGSTDDRI VVPQRGRSTR VPVASVLITRTRPLEQKLISEEDLAANDILDYKDDDDKV
528 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.