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SPIRE2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SPIRE2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G102047-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SPIRE2 (NM_032451), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1832 | Protein spire homolog 2 | Spir-2 | SPIR2 | SPIRE2 | Spire homolog 2 (Drosophila)
Origin Species
Human
NCBI GenBank
T75274
SwissProt
Q8WWL2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
79.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MARAGSCGGA AAGAGRPEPW ELSLEEVLKA YEQPLNEEQA WAVCFQGCRG LRGSPGRRLR
61DTGDLLLRGD GSVGAREPEA AEPATMVVPL ASSEAQTVQS LGFAIYRALD WGLDESEERE
121LSPQLERLID LMANNDSEDS GCGAADEGYG GPEEEEEAEG VPRSVRTFAQ AMRLCAARLT
181DPRGAQAHYQ AVCRALFVET LELRAFLARV REAKEMLQKL REDEPHLETP RAELDSLGHT
241DWARLWVQLM RELRRGVKLK KVQEQEFNPL PTEFQLTPFE MLMQDIRARN YKLRKVMVDG
301DIPPRVKKDA HELILDFIRS RPPLKQVSER RLRPLPPKQR SLHEKILEEI KQERRLRPVR
361GEGWAARGFG SLPCILNACS GDAKSTSCIN LSVTDAGGSA QRPRPRVLLK APTLAEMEEM
421NTSEEEESPC GEVTLKRDRS FSEHDLAQLR SEVASGLQSA THPPGGTEPP RPRAGSAHVW
481RPGSRDQGTC PASVSDPSHP LLSNRGSSGD RPEASMTPDA KHLWLEFSHP VESLALTVEE
541VMDVRRVLVK AEMEKFLQNK ELFSSLKKGK ICCCCRAKFP LFSWPPSCLF CKRAVCTSCS
601IKMKMPSKKF GHIPVYTLGF ESPQRVSAAK TAPIQRRDIF QSLQGPQWQS VEEAFPHIYS
661HGCVLKDVCS ECTSFVADVV RSSRKSVDVL NTTPRRSRQT QSLYIPNTRT LDFK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

714 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.