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SPHKAP Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SPHKAP Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all SPHKAP antibodies →
$725.00each
Catalog Number: LS-G109488-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SPHKAP (NM_030623), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
A-kinase anchor protein SPHKAP | SKIP | SPHKAP | KIAA1678
Origin Species
Human
SwissProt
Q2M3C7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
183.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDGNSLLSVP SNLESSRMYD VLEPQQGRGC GSSGSGPGNS ITACKKVLRS NSLLESTDYW
61LQNQRMPCQI GFVEDKSENC ASVCFVNLGV NKDECSTEHL QQKLVNVSPD LPKLISSMNV
121QQPKENEIVV LSGLASGNLQ ADFEVSQCPW LPDICLVQCA RGNRPNSTNC IIFEINKFLI
181GLELVQERQL HLETNILKLE DDTNCSLSSI EEDFLTASEH LEEESEVDES RNDYENINVS
241ANVLESKQLK GATQVEWNCN KEKWLYALED KYINKYPTPL IKTERSPENL TKNTALQSLD
301PSAKPSQWKR EAVGNGRQAT HYYHSEAFKG QMEKSQALYI PKDAYFSMMD KDVPSACAVA
361EQRSNLNPGD HEDTRNALPP RQDGEVTTGK YATNLAESVL QDAFIRLSQS QSTLPQESAV
421SVSVGSSLLP SCYSTKDTVV SRSWNELPKI VVVQSPDGSD AAPQPGISSW PEMEVSVETS
481SIPSGENSSR QPQSALEVAL ACAATVIGTI SSPQATERLK MEQVVSNFPP GSSGALQTQA
541PQGLKEPSIN EYSFPSALCG MTQVASAVAV CGLGEREEVT CSVAPSGSLP PAAEASEAMP
601PLCGLASMEL GKEAIAKGLL KEAALVLTRP NTYSSIGDFL DSMNRRIMET ASKSQTLCSE
661NVVRNELAHT LSNVILRHSI DEVHHKNMII DPNDNRHSSE ILDTLMESTN QLLLDVICFT
721FKKMSHIVRL GECPAVLSKE TIRRRETEPS CQPSDPGASQ AWTKATESSS SSPLSNSHNT
781SLVINNLVDG MYSKQDKGGV RPGLFKNPTL QSQLSRSHRV PDSSTATTSS KEIYLKGIAG
841EDTKSPHHSE NECRASSEGQ RSPTVSQSRS GSQEAEESIH PNTQEKYNCA TSRINEVQVN
901LSLLGDDLLL PAQSTLQTKH PDIYCITDFA EELADTVVSM ATEIAAICLD NSSGKQPWFC
961AWKRGSEFLM TPNVPCRSLK RKKESQGSGT AVRKHKPPRL SEIKRKTDEH PELKEKLMNR
1021VVDESMNLED VPDSVNLFAN EVAAKIMNLT EFSMVDGMWQ AQGYPRNRLL SGDRWSRLKA
1081SSCESIPEED SEARAYVNSL GLMSTLSQPV SRASSVSKQS SCESITDEFS RFMVNQMENE
1141GRGFELLLDY YAGKNASSIL NSAMQQACRK SDHLSVRPSC PSKQSSTESI TEEFYRYMLR
1201DIERDSRESA SSRRSSQIWT AGLLSPSLRS PVCHRQSSMP DSRSPCSRLT VNVPIKANSL
1261DGFAQNCPQD FLSVQPVSSA SSSGLCKSDS CLYRRGGTDH ITNMLIHETW ASSIEALMRK
1321NKIIVDDAEE ADTEPVSGGS PSQAEKCANR LAASRMCSGP TLLVQESLDC PRKDSVTECK
1381QPPVSSLSKT ASLTNHSPLD SKKETSSCQD PVPINHKRRS LCSREVPLIQ IETDQREACA
1441GEPEPFLSKS SLLEEAEGHS NDKNIPDVVR GGDTAVSACQ IHSDSLDTRD VPEAEASTEA
1501RAPDEAPNPP SSSEESTGSW TQLANEEDNP DDTSSFLQLS ERSMSELVEE KKILKGQSES
1561TEAPASGPPT GTASPQRSLL VINFDLEPEC PDAELRATLQ WIAASELGIP TIYFKKSQEN
1621RIEKFLDVVQ LVHRKSWKVG DIFHAVVQYC KMHEEQKDGR LSLFDWLLEL G
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1671 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.