LSBio
SPEF2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SPEF2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all SPEF2 antibodies →
$725.00each
Catalog Number: LS-G109722-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Sperm Flagellar 2(SPEF2) (NM_024867), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
209.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSEILCQWLN KELKVSRTVS PKSFAKAFSS GYLLGEVLHK FELQDDFSEF LDSRVSSAKL
61NNFSRLEPTL NLLGVQFDQN VAHGIITEKP GVATKLLYQL YIALQKKKKS GLTGVEMQTM
121QRLTNLRLQN MKSDTFQERL RHMIPRQTDF NLMRITYRFQ EKYKHVKEDL AHLHFEKLER
181FQKLKEEQRC FDIEKQYLNR RRQNEIMAKI QAAIIQIPKP ASNRTLKALE AQKMMKKKKE
241AEDVADEIKK FEALIKKDLQ AKESASKTSL DTAGQTTTDL LNTYSDDEYI KKIQKRLEED
301AFAREQREKR RRKLLMDQLI AHEAQEEAYR EEQLINRLMR QSQQERRIAV QLMHVRHEKE
361VLWQNRIFRE KQHEERRLKD FQDALDREAA LAKQAKIDFE EQFLKEKRFH DQIAVERAQA
421RYEKHYSVCA EILDQIVDLS TKVADYRMLT NNLIPYKLMH DWKELFFNAK PIYEQASVKT
481LPANPSREQL TELEKRDLLD TNDYEEYKNM VGEWALPEEM VDNLPPSNNC ILGHILHRLA
541EKSLPPRAES TTPELPSFAV KGCLLGKTLS GKTTILRSLQ KDFPIQILSI DTLVQEAIQA
601FHDNEKVSEV LPIQKNDEED ALPVLQEEIK ESQDPQHVFS AGPVSDEVLP ETEGETMLSA
661NADKTPKAEE VKSSDSFLKL TTRAQLGAKS EQLLKKGKSI PDVLLVDIIV NAINEIPVNQ
721DCILDGFPMT LNQAQLLEEA LTGCNRNLTE VERKKAQKST LAIDPATSKE IPLPSPAFDF
781VILLDVSDTS SMSRMNDIIA EELSYKTAHE DISQRVAAEN QDKDGDQNLR DQIQHRIIGF
841LDNWPLLEQW FSEPENILIK INAEIDKESL CEKVKEILTT EIAKKKNKVE KKLEEKEAEK
901KAAASLAELP LPTPPPAPPP EPEKEKEIHQ SHVASKTPTA KGKPQSEAPH GKQESLQEGK
961GKKGETALKR KGSPKGKSSG GKVPVKKSPA DSTDTSPVAI VPQPPKPGSE EWVYVNEPVP
1021EEMPLFLVPY WELIENSYIN TIKTVLRHLR EDQHTVLAYL YEIRTSFQEF LKRPDHKQDF
1081VAQWQADFNS LPDDLWDDEE TKAELHQRVN DLRDRLWDIC DARKEEAEQE RLDIINESWL
1141QDTLGMTMNH FFSLMQAELN RFQDTKRLLQ DYYWGMESKI PVEDNKRFTR IPLVQLDSKD
1201NSESQLRIPL VPRISISLET VTPKPKTKSV LKGKMDNSLE NVESNFEADE KLVMDTWQQA
1261SLAVSHMVAA EIHQRLMEEE KENQPADPKE KSPQMGANKK VKKEPPKKKQ EDKKPKGKSP
1321PMAEATPVIV TTEEIAEIKR KNELRVKIKE EHLAALQFEE IATQFRLELI KTKALALLED
1381LVTKVVDVYK LMEKWLGERY LNEMASTEKL TDVARYHIET STKIQNELYL SQEDFFINGN
1441IKVFPDPPPS IRPPPVEKEE DGTLTIEQLD SLRDQFLDMA PKGIIGNKAF TDILIDLVTL
1501NLGTNNFPSN WMHLTQPELQ ELTSLLTVNS EFVDWRKFLL VTSMPWPIPL EEELLETLQK
1561FKAVDKEQLG TITFEQYMQA GLWFTGDEDI KIPENPLEPL PFNRQEHLIE FFFRLFADYE
1621KDPPQLDYTQ MLLYFACHPD TVEGVYRALS VAVGTHVFQQ VKASIPSAEK TSSTDAGPAE
1681EFPEPEENAA REERKLKDDT EKREQKDEEI PENANNEKMS METLLKVFKG GSEAQDSNRF
1741ASHLKIENIY AEGFIKTFQD LGAKNLEPIE VAVLLKHPFI QDLISNYSDY KFPDIKIILQ
1801RSEHVQGSDG ERSPSRHTEE KK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1822 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.