Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SPECC1 / CYTSB Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G120931-100
Catalog Number: LS-G120931
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SPECC1 (NM_001033555), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CYTSB | Cytospin-B | HCMOGT-1 | SPECC1 | Structure protein NSP5a3b | Structure protein NSP5b3a | Structure protein NSP5b3b | Structure protein NSP5a3a | HCMOGT1 | NSP | NSP5 | Nuclear structure protein 5 | Sperm antigen HCMOGT-1
- Origin Species
- Human
- NCBI GenBank
- NM_001033554
- SwissProt
- Q5M775
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 110.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGNHSGRPED PEPGAFTTTK RTGIPAPREF SVTVSRERSV PRGPSNPRKS VSSPTSSNTP61TPTKHLRTPS TKPKQENEGG EKAALESQVR ELLAEAKAKD SEINRLRSEL KKYKEKRTLN121AEGTDALGPN VDGTSVSPGD TEPMIRALEE KNKNFQKELS DLEEENRVLK EKLIYLEHSP181NSEGAASHTG DSSCPTSITQ ESSFGSPTGN QLSSDIDEYK KNIHGNALRT SGSSSSDVTK241ASLSPDASDF EHITAETPSR PLSSTSNPFK SSKCSTAGSS PNSVSELSLA SLTEKIQKME301ENHHSTAEEL QATLQELSDQ QQMVQELTAE NEKLVDEKTI LETSFHQHRE RAEQLSQENE361KLMNLLQERV KNEEPTTQEG KIIELEQKCT GILEQGRFER EKLLNIQQQL TCSLRKVEEE421NQGALEMIKR LKEENEKLNE FLELERHNNN MMAKTLEECR VTLEGLKMEN GSLKSHLQGE481KQKATEASAV EQTAESCEVQ EMLKVARAEK DLLELSCNEL RQELLKANGE IKHVSSLLAK541VEKDYSYLKE ICDHQAEQLS RTSLKLQEKA SESDAEIKDM KETIFELEDQ VEQHRAVKLH601NNQLISELES SVIKLEEQKS DLERQLKTLT KQMKEETEEW RRFQADLQTA VVVANDIKCE661AQQELRTVKR KLLEEEEKNA RLQKELGDVQ GHGRVVTSRA APPPVDEEPE SSEVDAAGRW721PGVCVSRTSP TPPESATTVK SLIKSFDLGR PGGAGQNISV HKTPRSPLSG IPVRTAPAAA781VSPMQRHSTY SSVRPASRGV TQRLDLPDLP LSDILKGRTE TLKPDPHLRK SPSLESLSRP841PSLGFGDTRL LSASTRAWKP QSKLSVERKD PLAALAREYG GSKRNALLKW CQKKTQGYAN901IDITNFSSSW SDGLAFCALL HTYLPAHIPY QELNSQEKKR NLLLAFEAAE SVGIKPSLEL961SEMLYTDRPD WQSVMQYVAQ IYKYFETTRTRPLEQKLISEEDLAANDILDYKDDDDKV
987 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.